Dendritic spines are the postsynaptic compartments of excitatory neurotransmission. We combined electron microscopy and quantitative biochemistry with super-resolution microscopy to annotate more than 47,000 spines for more than 100 synaptic targets, resulting in a nanoscale 3D model of spines.
Nature Neuroscience 2021
We use super-resolution microscopy to determine the effect of Src kinase phosphorylation on the actin cytoskeleton .
Sci. Rep. 2018
Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells. We show here that the small dialdehyde glyoxal can not only successfully replace PFA but is more efficient.
EMBO Journal 2018
Protein copy numbers are typically estimated using biochemical approaches. We show here that combining imaging enables us to calculate precise copy numbers at biochemically untangible regions, such as subcellular organelles.
Sci. Rep. 2018
The function of endosomes and of endosome-like structures in the presynaptic compartment is still controversial.
Exp Cell Res. 2015